If you have only one specimen to study, just use a portion of the cap. The spores will fall on the paper, foil or glass, making a spore print pattern. Cover the cap with a paper cup or glass and leave for 2-24 hours, depending on the humidity and the freshness of the mushroom. Put a drop of water on the top of the cap to help release the spores.
Cut off the stem and place the cap, with the gills facing down, on a piece of aluminum foil, a white piece of paper, an index card or a glass microscope slide. Mushrooms with gills: The spores lie on the gill surface. Spore color can range from white to many other shades, including black. In order to see a group of spores and also the color of the spores, we can make a spore print. On a mature mushroom, many thousands of spores grow on just one gill or on a small piece of a mushroom. Mushroom spores are very small and can only be seen individually with a microscope.
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